Calibration Curve Calculator

Find concentration from a calibration line.

Concentration (x) 6

Formula: x = (signal − b) ÷ slope

Step-by-step with your numbers:

1. Values used:

2. Slope (m) = 0.05

3. Intercept (b) = 0.02

4. Measured signal (y) = 0.32

6. Concentration (x) = 6

Did we solve your problem today?

Use a standard curve (y = mx + b) to find an unknown concentration from a reading.

The math behind it

From y = mx + b, concentration x = (signal − intercept) ÷ slope.

slope 0.05, b 0.02, signal 0.32 → x = 6.

Where do m and b come from?

From linear regression of your standards (use the Linear Regression calculator).