qPCR Efficiency Calculator

Find PCR amplification efficiency from a standard-curve slope.

Efficiency (%) 100.08

Formula: E = (10^(−1/slope) − 1) × 100

Step-by-step with your numbers:

1. Values used:

2. Standard curve slope = -3.32

4. Efficiency = 100.08%

Did we solve your problem today?

Assess how well your qPCR doubles product each cycle.

The math behind it

E = 10^(−1/slope) − 1. A slope of −3.32 gives 100% efficiency (perfect doubling).

Slope −3.32 → 100%.

Acceptable range?

90–110% efficiency is generally considered good.