Biology

qPCR Efficiency Calculator

Find PCR amplification efficiency from a standard-curve slope.

Calculated Output

Efficiency (%) 100.08

Formula: E = (10^(−1/slope) − 1) × 100

Step-by-Step Breakdown

1. Values used:

2. Standard curve slope = -3.32

4. Efficiency = 100.08%

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The math behind it

E = 10^(−1/slope) − 1. A slope of −3.32 gives 100% efficiency (perfect doubling).

Slope −3.32 → 100%.

Acceptable range?

90–110% efficiency is generally considered good.